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What is the difference between a CBC with a manual differential and a automated differential?
Wiki User
∙ 12y ago
Most analysers provide an automated differential with the white cell count, red cell count, and indices. If for some reason, the differential is rejected, or flagged on the automated differential provided, the differential must be performed manually by properly trained technologists. A manual differential involves making a smear on a glass slide and staining the slide with Wright's stain which is a combination of acid and basic dyes. The stain results in white blood cells staining blue and the red blood cells staining red, platelets also appear blue. 100 white blood cells are counted under a microscope-dividing them into the 5 white cell types and evaluating the cells for abnormalities. The red cells are observed for size, shape, and hemoglobin content. The number of platelets is estimated as well. The absolute number of each white cell line is then reported using the percentage of each cell type and with white cell count.
Wiki User
∙ 12y ago
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