What is the resolution power of a transmission electron microscope?

Answer 1

The Transmission Electron Microscope (TEM) was the first type of Electron Microscope to be developed and is patterned exactly on the Light Transmission Microscope except that a focused beam of electrons is used instead of light to "see through" the specimen. It was developed by Max Knoll and Ernst Ruska in Germany in 1931.

The first Scanning Electron Microscope (SEM) debuted in 1942 with the first commercial instruments around 1965. Its late development was due to the electronics involved in "scanning" the beam of electrons across the sample. TEM focus a beam of electrons through a specimen while SEM focus a beam of electrons onto the surface of a specimen and the image provided is 3-D
the transmission microscope magnifies 300,000 more times and the scanning microscope only magnifies 100,000 more the transmission gives the image of the inside and the scanning microscope gives a 3D image of the surface of the specimen

Answer 2

Scanning electron microscope

Transmission electron microscopy

A scanning electron microscope (SEM) is a type ofelectron microscope that images a sample by scanning it with a high-energy beam of electrons in araster scan pattern.

Transmission electron microscopy (TEM) is amicroscopy technique whereby a beam ofelectrons is transmitted through an ultra thin specimen, interacting with the specimen as it passes through.

• Pass a beam of electrons over the surface of the specimen in the form of a 'scanning' beam.

• Pass a beam of electrons through the specimen.

can magnify a sample up to 100,000 times.

) can magnify a sample up to one million times.

A sharply focused electron beam moves over the sample to create a magnified image of the surface.

An electron beam is directed onto the sample to be magnified and some of the electrons pass through and form a magnified image of the specimen.

can produce three-dimensional images of the surface of objects.

Can produce two-dimensional image of the surface of objects.

· The "Virtual Source" at the top represents theelectron gun, producing a stream of monochromatic electrons.

The "Virtual Source" at the top represents theelectron gun, producing a stream of monochromatic electrons.

The elements and compounds the sample is composed of and their relative ratios, in areas ~ 1 micrometer in diameter.

The arrangement of atoms in the specimen and their degree of order, detection of atomic-scale defects in areas a few nanometers in diameter.

scanning microscope can able to scan outer cell wall, membrane etc

transmission microscope can help to study the interior structure of microbes.

Answer 3

Scanning Electron Microscopy (SEM) is typically used for observing surface details and produces a three-dimensional effect.

Transmission Electron Microscopy (TEM) is used to observe fine details of cell structure, like the number of layers that envelop a cell. This process requires EXTREME and elaborate specimen preparation.

The ability to resolve is to separate two objects very close together. Technically they resolve the same, except in SEM electrons scan back and forth over an object whereas TEM transmits electrons through an object.

Answer 4

The resolving power of the transmission electron microscope is 100 times better than the resolving power of the scanning electron microscope. The resolving power of the compound light microscope is 1000 times better than the resolving power of the unaided eye.

Answer 5

A transmission electron microscope looks at the outside membrane of cells. The electronic beam goes through the tissue. You can tell the difference in thin and thick areas of the cell.

A scanning electron microscope looks at the interior of cells. There is a gold part in the microscope that is a reflective substance that shoots electronic beams through toward the cell.