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Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
Sodium acetate buffer helps by reacting with the membrane protein and precipitating them, thus facilitating the dna isolation.
Tris is here to maintain pH
what is the function of sorbitol in producing plant protoplasts
To remove divalent cations(mg2+) required by nucleAses
Boric Acid is an extraction buffer used in the isolation of DNA and when it is employed with a correct pH then it can help in getting rid of the cell components without disturbing the cell organelles i.e it retains the organelles.
Sodium ions neutralize the negative charge of the DNA backbone (phosphates) making the DNA less hydrophilic (less likely to be solubilized) in the wash solution
pH control. In other words, buffering of lysing and extracting matrix to preserve DNA integrity, otherwise compromised in extreme pH.
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.
it act as as a cationic detergent for the isolation dna from the given sample
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.