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Q: What is the function of chloroformisoamyl in DNA isolation?
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Role of sucrose in DNA isolation from human blood?

Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood


What are the function of sodium acetate in dna isolation?

Sodium acetate buffer helps by reacting with the membrane protein and precipitating them, thus facilitating the dna isolation.


Function of tris HCl in DNA isolation?

Tris is here to maintain pH


What is the role of sorbitol in DNA isolation?

what is the function of sorbitol in producing plant protoplasts


What is the function of ethylene diamine tetraacetic acid in DNA isolation?

To remove divalent cations(mg2+) required by nucleAses


What is the function of boric acid in isolation of DNA?

Boric Acid is an extraction buffer used in the isolation of DNA and when it is employed with a correct pH then it can help in getting rid of the cell components without disturbing the cell organelles i.e it retains the organelles.


What is the function of sodium citrate in DNA isolation?

Sodium ions neutralize the negative charge of the DNA backbone (phosphates) making the DNA less hydrophilic (less likely to be solubilized) in the wash solution


What is the function of the phosphate buffer in DNA isolation?

pH control. In other words, buffering of lysing and extracting matrix to preserve DNA integrity, otherwise compromised in extreme pH.


Use of agrose in DNA isolation?

Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size


What is the principle involved in the isolation procedure?

DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.


What is role of Cetyl trimethyl ammonium bromide in DNA isolation?

it act as as a cationic detergent for the isolation dna from the given sample


Role of phenol chloroform in DNA isolation?

phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.