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In a M13 phage we can insert about 1-4 kb of target DNA sequence which is its maximum insert capacity.

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13y ago
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13y ago

About 1-5 kb (kilo base) can be inserted in a plasmid vector.

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In P1 artificial chromosomes (PAC) vectors you can add about 100-300 kb of foreign DNA.

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13y ago

The insert capacity of a bacterial artificial chromosome (BAC) is about less than or equal to 300 kb.

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The maximum numbers of bases from a foreign DNA that can be inserted in a Yeast artificial chromosome (YAC) is 0.2 million- 2 million bases

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Q: What is the insert capacity of PACs vectors in DNA cloning?
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