- "Ace of Spades" by Motörhead
- "Bark at the Moon" by Ozzy Osbourne
- "Cochise" by Audioslave
- "Cowboys from Hell" by Pantera
- "Crossroads" by Cream
- "Fat Lip" by Sum 41
- "Frankenstein" by The Edgar Winter Group
- "Godzilla" by Blue Öyster Cult
- "Heart Full of Black" by Burning Brides
- "Hey You" by The Exies
- "Higher Ground" by Red Hot Chili Peppers
- "I Love Rock and Roll" by Joan Jett and the Blackhearts
- "I Wanna Be Sedated" by The Ramones
- "Infected" by Bad Religion
- "Iron Man" by Black Sabbath
- "Killer Queen" by Queen
- "More Than a Feeling" by Boston
- "No One Knows" by Queens of the Stone Age
- "Sharp Dressed Man" by ZZ Top
- "Smoke on the Water" by Deep Purple
- "Spanish Castle Magic" by Jimi Hendrix
- "Stellar" by Incubus
- "Symphony of Destruction" by Megadeth
- "Take It Off" by The Donnas
- "Take Me Out" by Franz Ferdinand
- "Texas Flood" by Stevie Ray Vaughan
- "Thunder Kiss '65" by White Zombie
- "Unsung" by Helmet
- "You Got Another Thing Comin'" by Judas Priest
- "Ziggy Stardust" by David Bowie
- "All of This" by Shaimus
- "Behind the Mask" by Anarchy Club
- "The Breaking Wheel" by Artillery
- "Callout" by The Acro-Brats
- "Caveman Rejoice" by The Bags
- "Cheat on the Church" by Graveyard BBQ
- "Decontrol" by Drist
- "Eureka, I've Found Love" by The Upper Crust
- "Even Rats" by The Slip
- "Farewell Myth" by Made in Mexico
- "Fire It Up" by Black Label Society
- "Fly on the Wall" by Din
- "Get Ready 2 Rokk" by Freezepop
- "Guitar Hero" by Monkey Steals the Peach
- "Hey" Honest Bob and the Factory-to-Dealer Incentives
- "Sail Your Ship By" by Count Zero
- "Story of My Love" by The Model Sons
Two other songs are present on the game media, but they can only be unlocked through the use of a PlayStation 2 cheat device, such as GameShark, Code Breaker or Action Replay.
- "Graveyard Shift" - artist unknown
- "Trippolette" - by Andrew Buch, a composer at Berklee College of Music who has worked on the Harmonix team