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What is the main difference between a coomassie stain and a western blot?
Wiki User
∙ 13y ago
In coomassie staining, a dye binds to all proteins, visualizing all of them. In this way, it's nonspecific. The dye diffuses into the the original gel.
In a western, an antibody binds specifically to one or more proteins, making it specific for proteins to which the antibody can bind. In order for the antibody to access the proteins from the original gel, they are moved (blotted) to the surface of a membrane (usually nitrocellulose).
Wiki User
∙ 13y ago
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