answersLogoWhite

0


Best Answer

The eukaryotic chromosomes are larger in size. Hence in order to overcome the limitation of DNA synthesis, multiple origins of replication are present in order to complete replication in a reasonable period of time.

  • At the replication origin the strands of DNA must dissociate and unwind in order to allow access to DNA polymerase.

  • Unwinding of the duplex at the origin as well as along the strands as the replication process proceeds is carried out by helicases.


  • The resultant regions of single-stranded DNA are stabilized by the binding of single-strand binding proteins.

  • The stabilized single-stranded regions are then accessible to the activities of other enzymes required for replication to proceed.


  • The site of the unwound template strands is termed the replication fork.

In order for DNA polymerases to synthesize DNA they must encounter a free 3'-OH which is the substrate for attachment of the 5'-phosphate of the incoming nucleotide.

During replication the 3'-OH is supplied through the use of an RNA primer, synthesized by the primase activity. The primase utilizes the DNA strands as templates and synthesizes a short stretch of RNA generating a primer for DNA polymerase.

Synthesis of DNA proceeds in the 5'---->3' direction through the attachment of the 5'-phosphate of an incoming deoxy nucleosidetriphosphate (dNTP) to the existing 3'-OH in the elongating DNA strands with the concomitant release of pyrophosphate.

Initiation of synthesis, at origins of replication, occurs simultaneously on both strands of DNA.

DNA synthesis process then proceeds bidirectionally, with one strand in each direction being copied continuously and one strand in each direction being copied discontinuously.

The experimental support for this bidirectional mode of replication in the cells of higher eukaryotes was acquired by the fiber autoradiography of labeled DNAs got from mammalian cell cultures. When the replicating DNA extracted and isolated from eukaryotic cells are examined under the electron microscope, the clear "bubble" like structures extending from multiple replication origins are clearly visible.

source: www.examville.com

User Avatar

Wiki User

14y ago
This answer is:
User Avatar
More answers
User Avatar

Wiki User

9y ago

DNA is a double helix. So during replication it unwinds and separates into two strands. One strand will be called the non-coding and the other will be called the coding strand. The non-coding strand will be the template for the new one.

This answer is:
User Avatar

User Avatar

Wiki User

11y ago

The DNA splits up along the branches between the chains as a result of DNA helicase. Then free, single monomer units bind to their respective matches (A to T and C to G units) and reform to create two identical chains of DNA. The DNA monomer units are bound to the parent DNA strands using DNA Polymerase.

In industry this process is copied to produce large amounts of a sample of DNA. it is often during crime investigations to produce large amounts of a small sample.

This answer is:
User Avatar

User Avatar

Wiki User

11y ago

Works like a negetive and it uses the negetive of DNA to make more DNA

This answer is:
User Avatar

User Avatar

Wiki User

12y ago

One strand of the DNA molecule

This answer is:
User Avatar

Add your answer:

Earn +20 pts
Q: What serves as the template for DNA replication?
Write your answer...
Submit
Still have questions?
magnify glass
imp
Related questions

What Features of DNA replication make it semi-conservative?

the original strand serves as a temple for the new molecule.


What acts as the origianl template in DNA replication?

A strand of DNA


Semiconservative replication involes a template what is the template?

one strand of the DNA molecule


What enzyme reads the DNA template?

Various DNA polymerases read the DNA template during replication of DNA. Various RNA polymerases read the DNA template during transcription.


How is retrovirus different from DNA virus?

Retro virus has reverse transcription in its replication cycle. In other words, rna is template for synthesis of dna. With dna virus, there is no reverse transcription in the replication cycle. Dna is the template for dna synthesis.


What is the process in which the DNA double helix unfold and each strand serves as a template for the synthesis of a new strand?

I believe it's called replication. not really sure though...


What is the explanation for the process of replication?

During DNA replication, the DNA molecule separates into two strands, then produces two new complementary strands following the rules of base pairing. Each strand of the double helix of DNA serves as a template, or model, for the new strand.


RNA molecule that is template for translation?

DNA serves as a template for transcribing mRNA.


What is the process of DNA replication?

Double Helix unwound and each strand acts as a template. Bases are matched to synthesize the new strand.the DNA molecule splits down the middle, or unzips, into two strands.Veach strand serves as a template or model, to produce the new strands.Vtwo new DNA strands are produced, following the rules of base pairing.a. replication


What is compatible to TCGTA in a sequence template in DNA replication?

AGCAT


What does semi-conservative mean regard to DNA replication?

replicated DNA is made of one old strand and one new strand.


How is DNA copied semi-conservative replication?

The original DNA molecule is the template for the new DNA molecules.