Lactophenol Cotton Blue is recommended for mounting and staining yeast and molds. It is formulated with lactophenol, which serves as a mounting fluid, and cotton blue. Organisms suspended in the stain are killed due to the presence of phenol. The high concentration of the phenol deactivates lytic cellular enzymes thus the cells do not lyse. Cotton blue is an acid dye that stains the chitin present in the cell walls of fungi.
el azul de lactofenol tiene tres funciones importantes a la hora de observar hongos del tipo mohos optenidos por aislamiento o de medios inoculados, el fenol destruye la flora acompañante, el acido lactico conserva las estructuras fungicas al provocar un cambio de gradiente osmotico con relacion al interior del fungico generando una pelicula por asi llamarlo protectora, pero el que realmente permite apreciar la estructura fungica es el azul de algodon el cual posee la capasidad de aderirse a la quitina presente en las hifas y conidios de los hongos microscopicos y tambien los macro pero es fundamentalmente utilizado en microscopicos u hongos inperfectos como aspergilius o penicillium
http://www.lukor.com/foro/1684.htm
translated by me:
lactophenol blue has 3 important functions when observing filamentous fungi. Phenol destroys contaminants compounds that has the fungi, the acid lactic conserves the fungi structures by provoking osmotic gradient change with relation of the interior of the fungi forming a protective "layer" (not really a layer i just could find the correct word to translate the other one..sorry I'm doing my best). The cotton blue is the one that gives the color and permit to visualize the fungi under optic microscope. it adheres to the chitin of the fungi walls of hiphas and conidios.
just to clarify: phenol + lactic acid + cotton blue = lactophenol cotton blue staining.
For the main part I would consider using the stain for fungal spores, particularly ones that are translucent or nearly so. Likewise, I use prefer Congo Red (if needed) to enhance cells within the actual fungus itself. RM
According to some Medical Technologists, LPCB is best and most commonly used in mycology department. LPCB is placed on an uncontaminated slide BEFORE placing the fungal culture because of that cultured fungi are alive and they needed to be killed as soon as possible to avoid possible abnormal side effect/s such as infection. As you can see, lactic acid or lacto preserves organisms while phenol kills organisms that would lead to arousal and isolation of fungal elements. With it's blue physical characteristic, it is easier to see spores or hyphae thus easier to identify presence of fungal elements.
Phenol will kill any live organisms; lactic acid which preserves fungal structures, and cotton blue which stains the chitin in the fungal cell walls.
Staining of fungus by using lactophenol cotton blue. Where lactophenol acts as a cleaning agent and lactic acid acts as a preservative.
It allows you to visualize the hyphae. (I think it works by staining the chitin in the cell walls.)
the same
Some fungi are parasites and some parasites are fungi, but many fungi are not parasites and many parasites are not fungi.
Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism.
Cotton is not as warm or as water repellent as other fabrics.
Using a microscope. Movement or lack of is easily noted visually, especially after staining.
Avoid staining sheets or pants during periods by using adira period panty.These are very cotton and comfy.Safe to use by younger girls too.
my brother has a fungi in his feet
the same
you have to insert the cotton
Some fungi are parasites and some parasites are fungi, but many fungi are not parasites and many parasites are not fungi.
the economic important of a fungi is how they using asexual repruducing :)
using a copper derivate product
One grade of paper is known as cotton card; there is a game named cotton card; cotton fibre is aligned using a cotton carder.
Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism.
Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism.
The sheets were as soft as cotton.
Fungi are decomposers. They absorb food by breaking down another organism and using its nutrients. Fungi are heterotrophs.