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The first items you should collect are the serum or clot tubes. Then, follow this by other plasma tubes, then purple (EDTA), then blue (NA Citrate). The reason for this is that during the needle insertion, tissue substances (tissue thromboplastin, electorlytes, etc...) will enter the lumen of the needle and be aspirated into the tube initially. When performing certain tests (clotting studies, etc...) these trace substances may interfere with the results although that is really splitting hairs. So, best to clear the needle with clot tubes, then draw the others.

Actually, the order of draw has changed....blood culture, blue (sodium citrate), serum or sst gel tubes (plain red, gold sst), heparin-sodium or lithium(light green, dark green), EDTA (purple), then gray(used for glucose screening).

Actually it's:

Here is the current order of draw according to CLSI standard H3-A6 is as follows.This should ALWAYS be followed to prevent erroneous results due to additive crossover:

1.) Blood cultures (yellow) SPS (sterile)

2.) Light blue (buffered sodium citrate tube)

3.) Red (plain), or Tiger-Top mottled red (gel separator tube)

4.) Green heparin and light green (sodium or lithium with or without separators)

5.) Lavender (EDTA)

6.) Pink, white, or royal blue (EDTA)

7.) Gray (Na flouride/potassium oxalate)

8.) Dark blue (FD

Hi I am taking phlebotomy and I agree with your answer but I would like to let you know what I have been taught-my teacher has been teaching/been a phlebotomist for 27 years.She taught us that the order of draw is:

1.) YELLOW: Additive= SPS and Broth

2.) LIGHT BLUE: Additive= 32% buffered,Na citrate

3.) PLAIN RED: Additive= Nothing

4.) SST(or 'tiger top' tube top tube): Additive= Clot activator

5.) GREEN: Additive= Heparin,Na Lithium

6.) LAVENDER: Additive= EDTA

7.) GRAY: Additive= Oxalates

8.) ROYAL BLUE: Additive= See labels,green heparin,plain red and lav.

9.) YELLOW (tan): Additive= ADC and A/B

I just Graduated from phlebotomy school, Jan 2011 and I was taught the Current Order of Draw is:

Blood Cultures/SPS/Sterile

(Plasma) Light Blue- Sodium Citrate *

(Serum) Red- Plain "No Additive

(Serum) Gold- SST= Serum Separator tube

(Plasma) Light Green- PST- Lithium Heparin

(Plasma) Dark Green= Sodium Heparin *

(Plasma) Lavender/Pink- EDTA K2-EDTA *

(Plasma) Gray- Sodium Floride and Potassium Oxalate*

ALL OTHERS

*= Anticoagulant Tubes Rim Plasma when centrifuged

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13y ago
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10y ago

they draw blood by 6 different techniques

Evacuated

Syringe

Butterfly

Glucose Dermal Puncture

Bleeding Time

Capillary Stick

Resources : National Certification Career Association NCCA

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16y ago

It is the process of drawing/collecting blood (samples).

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11y ago

lavender,green,blue,yellow,red

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Q: The phlebotomy order of draw is reversed when blood is collected with a syringe?
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