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What is the purpose of the alcohol in the strawberry DNA extraction lab?
Wiki User
∙ 12y ago
Hmm, I think it's used to wash the DNA from the substance used for extracting the DNA, such as Isopropanol, CLS, NLS, PPS which are used in the process of extraction to the process to precipitate the DNA (using isopropanol). DNA is insoluable in alcohol.
Actually the real reason for using alcohol in a DNA extraction is to "precipitate" (NOT RAIN) the DNA, as you may have realized when you did the experiment , the Alcohol step is the last step that brings the DNA to the surface.
Finally alcohol can be added so that the DNA can be seen, but first it is crucial to know why the addition of alcohol is such an important step. This step is closely related to a process called precipitation, which is " the process of separating a substance from a solution as a solid" (answers.com, precipitation). Precipitation separates the DNA so that it can no longer remain dissolved and so that it can be seen. DNA is less dense than water and alcohol , so the DNA rises to the alcohol layer. Also the DNA is soluble in water and insoluble in alcohol, so when it is just in the mixture it is quite invisible but once the alcohol is added it condensates by precipitation to the point where it is very visible.
Wiki User
∙ 13y ago
Wiki User
∙ 14y agoDNA molecules are surrounded by water molecules when in solution. This surrounding water is called the shell of hydration. Alcohol is a dehydrating agent. When alcohol is added (along with certain salts) during DNA isolation, it results in the disruption of the hydration shell.
At this stage, the DNA comes out of solution and is said to be in a precipitated state. Precipitated DNA can then be separated from the remaining solubilized components through s process called centrifugation.
Wiki User
∙ 9y agoAccording to me, we use alcohol because DNA is insoluble in alcohol, it aggregates together, giving a pellet in centrifugal and we can see a precipitated DNA with naked eyes (that we suppose to see in experiment i.e DNA extraction)....
Wiki User
∙ 12y agoSo that scientists can make strawberry daiquiris, duh! :P
If you're being serious, the strawberry gets made into a liquid and prepped to separate the DNA, alcohol is less dense than water so it helps capture the DNA.
Wiki User
∙ 11y agoIsopropyl alcohol can really damage the body's lungs if someone drinks it. If you rub it on your body you at 1st feel a little better but in 24-48 hours your whole body well feel as though you had just ran into some fire
Wiki User
∙ 13y agoBecause the cold alcohol reduces the solubility (ability to dissolve) of DNA. It makes the DNA stay intact while the proteins and lipids dissolve or break down.
Wiki User
∙ 10y agoAlcohol is added to the aqueous solution of DNA at the end point of the extraction process, it precipitates out the DNA as DNA is insoluble in alcohol.
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What are the precaution while conducting DNA extraction?
Perhaps the most basic precaution is to make sure that the warm water bath that will be used has a temperature that doesn't exceed 90C so as to denature only the protein component of crude extract and not the DNA which is the target isolate.
How do the enzymes pectinase and cellulase aid in the extraction of DNA from strawberries?
In a strawberry to extract the DNA it is required to break down the cell membrane (both the membrane protecting the cell as well as the membrane protecting the nucleus) and pectinase and cellulase in strawberries are enzymes that are break down these membrane...at least in a strawberry.
How could you determine what percentage of a strawberry's mass is DNA?
Divide the weight of the strawberry's DNA by the strawberry itself.
What does sodium citrate do in DNA extraction?
how to make sodium citrate in 10% ethanol for DNA extraction
What is DNA extraction?
There are three basic steps in a DNA extraction, the details of which may vary depending on the type of sample and any substances that may interfere with the extraction and subsequent analysis. * Chelate divalent cations such as Mg2+ and Ca2+ to stop dnase enzymes functioning and degrading the DNA * Break open cells by grinding or sonication, and remove membrane lipids by adding a detergent. * Precipitate DNA in cold ethanol or isopropanal, DNA is insoluble in alcohol and clings together; this step also removes salt.
What is the purpose of soap in strawberry extraction?
The purpose is to help the mixture of salt water and ethanol so the can find the DNA of strawberry bananna etc. Extrsctions
What is the purpose of alcohol when extracting DNA?
According to me, we use alcohol because DNA is insoluble in alcohol, it aggregates together, giving a pellet in centrifugal and we can see a precipitated DNA with naked eyes (that we suppose to see in experiment i.e DNA extraction)....
Role of phenol chloroform isoamyl alcohol in plasmid DNA extraction?
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
What is the function of CL buffer in DNA extraction?
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
Why grind the liver in a DNA extraction lab?
the purpose of grinding any substance during dna extraction is cell loosening.
Can iso amyl alcohol replace phenol in DNA extraction?
No
What is the function of the alcohol in DNA extraction?
DNA is not soluble in isopropyl alcohol. It will precipitate out when you add this solvent. Once out of solution you can centrifuge it down and collect the pellet of DNA.
What is the purpose of using soap in a DNA extraction buffer?
The word detergent is used instead of soap in a DNA extraction buffer. Detergent is used to create a hydrophobic environment that favors the precipitation of proteins. Proteins are one of two major contaminants in DNA extraction (the other major contaminant being RNA). When protein precipitate, they can be separated by centrifugation and the DNA isolation procedure can continue.
Role of saline tris edta in DNA extraction?
Its purpose is to isolate DNA from a protein mixture.
What is the purpose of incubation in DNA extraction?
This is to give a period of time for the DNA to grow by replication; this allows there to be enough of a sample of DNA to extract.
What is the function in the following reagents used in the extraction and precipitation of DNA?
to extract the DNA from the solution, isopropyl alcohol is mixed with the DNA solution to precipitate the same.
