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What is the purpose of crystal violet in the Gram's stain procedure?
Wiki User
∙ 13y ago
Its the primary stain of the procedure. IT stains the Gram positive organisms
Wiki User
∙ 13y ago
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What happens if grams iodine is not applied after staining with crystal violet?
If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
What color does the gram positive stain?
It will hold the primary stain of violet.
Can grams iodine be used before primary stain?
Probably not. The CVI complex formed between the iodine and crystal violet will be trapped in the lipid layer and not the peptidoglycan layer, so you would probably end up over decolorizing. This would cause a lot of false gram negatives. Probably not. The CVI complex formed between the iodine and crystal violet will be trapped in the lipid layer and not the peptidoglycan layer, so you would probably end up over decolorizing. This would cause a lot of false gram negatives.
What is mordant used in the gram staining procedure and its function?
A mordant is an element which aids the chemical reaction that takes place between the dye and the fibre so that the dye is absorbed. It may also mean incisive or insistent especially in questioning something.
Why would gram negative bacteria appear purple after gram stain?
The simple staining procedure makes to visualize bacteria clearly, but it does not distinguish between organisms of similar morphology. The Gram staining method is named after the Danish bacteriologist (1882) Hans Christian Gram, is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram's method is crystal violet. Crystal violet is sometimes substituted with methylene blue, which is equally effective. The microorganisms that retain the crystal violet-iodine complex appear purple brown under microscopic examination. These microorganisms that are stained by the Gram's method are commonly classified as Gram-positive or Gram non-negative. Others that are not stained by crystal violet are referred to as Gram negative, and appear red. In this method the fixed bacterial smear is subjected to the following staining reagents in the order of sequence listed below Primary stain Crystal violet is used first and stains all cell purple = Mordant = Grams iodine serves as a mordant, a substance that increases the cell affinity for a stain. It does this by binding to the primary stain thus forming an insoluble complex. The resultant crystal violet iodine complex serves to identify the color of the stain. At this point all cells will appear purple black. = Decolorizing agent = Gram staining is based on the ability of bacteria cell wall to retaining the crystal violet dye during solvent treatment. The cell walls for Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than gram-negative bacteria In Gram-negative cells, subsequent treatment with a decolorizer, (95% ethyl alcohol) dissolves the lipid layer from the gram-negative cells. The removal of the lipid layer enhances the leaching of the primary stain from the cells into the surrounding solvent. In contrast, the solvent dehydrates the thicker Gram-positive cell walls, closing the pores as the cell wall shrinks during dehydration. As a result, the diffusion of the violet-iodine complex is blocked, and the bacteria remain stained. Counter stain A counterstain of basic fuchsin or saffranin is applied to the smear to give decolorized gram-negative bacteria a pink color. Since only gram-negative cells undergo decolorization they may now absorb the counter stain. Gram-positive cell retain the purple color of the primary stain. Basic fuchsin stains many Gram-negative bacteria more intensely than does safranin, making them easier to see. Some bacteria which are poorly stained by safranin, such as Haemophilus spp., Legionella spp., and some anaerobic bacteria, are readily stained by basic fuchsin, but not safranin. The polychromatic nature of the gram stain enables determination of the size and shape of both Gram-negative and Gram-positive bacteria. If desired, the slides can be permanently mounted and preserved for record keeping.
What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
What happens if grams iodine is not applied after staining with crystal violet?
If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
How many grams are in a 50000 kg crystal?
50,000kg = 50,000,000 grams
How is crystal-meth sold?
To dealers- ounces. To users- grams, half grams, quarter grams. Seewhere this is going.
What does an eight ball of crystal meth weigh?
The term "eight ball" refers to a quantity of cocaine or crystal meth that is equal to an eighth of an ounce, which is equivalent to 3.5 grams.
How many grams 1.333 cups all purpose flour?
That is approximately 178 grams
What is the gram staining procedure?
the gram staining procedure involves four basic steps; 1.the smear is first flooded wit the primary stain crystal violet dye.in this case the primary stain is the first dye applied in any multicelled staining procedure and it stains all the cells. 2.the smear is rinsed with water to remove any excess crystal violet and then its flooded wit a dilute solution of iodine called grams iodine.iodine acts as a mordant.i.e. the substance that increases the interaction and affinity of cellular components for a dye.this is done so that the cells can stain more strongly. in this case the iodine complex thereby decreases the solubility of the dye within the cells 3.the stained smear is rinsed again and then 95% alcohol or a mixture of alcohol and acetol is briefly added.this solvent acts as decolorizing agents and they readily remove the dye iodine complex from gram negative but not from gram positive bacteria. 4.a counter stain is then applied to import a contrasting color to the now colorless gram negative bacteria.for this purpose,the red dye safrannin is used.this dye stains gram negative as well as gram positive bacteria but because the gram positive are already stained purple,it impacts little difference
How much does a grams of Tina crystal shard cost?
15-40 us dollars
List the steps of gram staining procedu in order omit washings and list colors of gram positive and gram negative for staphylococcus epedermidis Bacillus subtilis Escherechia coli?
step 1 crystal violet step 2 grams iodine step 3 ethanol step 4 safranin
120 grams of flour in millilters?
120 grams of all-purpose flour = 285.7 milliliters.
B-ball of crystal meth?
Do you mean a "8- Ball" of meth... that's 3.5 grams
How many teaspoons in 15 grams of purposes flourer?
how many teaspoon are in 15 grams of purpose flourer
