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A mordant is an element which aids the chemical reaction that takes place between the dye and the fibre so that the dye is absorbed.

It may also mean incisive or insistent especially in questioning something.

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12y ago
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15y ago

Iodine solution is used as mordant in grams staining. At first smear is stained with crystal violet and then with iodine. When gram positive bacteria are treated with ethanol, the alcohol is thought to shrink the pores of thick peptidoglycan. Thus the dye-iodine complex is retained during this short decolorization step and the bacteria remain purple. In contrast, the gram negative get decolourized and when counterstain with saffranine it shows pink colour. So iodine from comlex between cell wall of gram positive and crystal violet.

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14y ago

A mordant is a substance used to set dyes on fabrics or tissue sections by forming a coordination complex with the dye which then attaches to the fabric or tissue. It may be used for dyeing fabrics, or for intensifying stains in cell or tissue preparations. In Histology, mordants are indispensible in adhering dyes to tissues for microscopic examination.

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11y ago

Mordant allow dyes to stay long in the shirt, even after washing. It is good to soak your shirt in sodium carbonate solution, before dying your shirt.

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11y ago

the purpose of a mordant in the gram stain ' to make the flagella visible

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10y ago

A mordant is a substance which is used to set dyes on fabrics or tissues. It can be used on dyeing fabrics or for stains to go on a quicker rhythm in cell and tissue preparations.

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Q: What is mordant used in the gram staining procedure and its function?
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Why is Iodine used in Gram staining?

Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.


What is the function of iodine solution in the gram stain?

It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.


What are the reagents and steps min a Gram stain?

The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.


Why gram staining is classified as differential staining?

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining


What is the appearance of gram negative bacteria after addition of mordant in the gram stain?

Red/pink

Related questions

What would be the effect of removing the mordant from the gram's staining procedure?

there is no real effect


Function of Gram's iodine?

Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.


What is the mordant in a gram stain?

Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.


Why is Iodine used in Gram staining?

Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.


What is the four staining procedure in bacteriology?

Gram, Ziel-Nielsen


Function of iodine in staining?

iodine act as a mordant..on the gram positive bacteria which got really thick and abundence of peptidoglycan layer, the crystal violet will fix to the peptidoglycan layer..meanwhile in gram negative bacteria which is lack of peptidoglycan layer, the alcohol or acetone will wash it away


What is the function of iodine solution in the gram stain?

It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.


Why is it important to follow the gram staining procedure?

This is simply important in order to have accurate staining results. If this is not followed, the process of the staining will result to false positives or false negatives.


What are the reagents and steps min a Gram stain?

The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.


What is crystal violet iodine complex?

In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .


Why gram staining classified as differential staining?

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining


Why is Gram staining classified as differential staining?

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining