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UV cut off of acetone is 330 nm and it is easyly available,non expensive. you can use any other solvent which has UV cut off above 250.
Wiki User
∙ 12y ago
suresh kumar
Wiki User
∙ 12y agoIn the laboratory, acetonitrile is used as a medium-polarity solvent that is miscible with water and has a convenient liquid range. With a dipole moment of 3.84 D, acetonitrile dissolves a wide range of ionic and nonpolar compounds and is useful as a mobile phase in HPLC and LCMS.
Wiki User
∙ 13y agoBecause permanent inks are acetone soluble.
Wiki User
∙ 10y agoAnonymous
You can use any other solvent which have uv cutoff range is above 250nm.
Acetone have uv cut off range is 330nm.
Easily available in market.
Non expensive.
suresh kumar
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
When calibration required?
Calibration is required during installation of new instrument to check whether the instrument works as per specifications or not. Calibration is required when instrument is shifted from one location to another. Calibration of instrument is done when instrument is used again after maintenance. Some instruments such as weighing balance are calibrated daily while some instruments or equipments such as HPLC, GC, etc., are calibrated or validated at specified interval as per standard operating procedure (SOP).
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
When are calibration standards used?
Calibration standards are used to record the accuracy of certain calibrations that are performed. The accuracy allows the user to find out how good the calibration was.
Why is Erbium perchlorate is used in calibration of HPLC?
Erbium has a strong absorption in uv and visible range, It is used in HPlc calibration for the wavelength accuracy verification of the PDA detector.
Why propyl paraben used in HPLC calibration?
propyl paraben being a stable compound with reproducible chromatographic parameters used as a internal standard for the calibration of the HPLC systems
Why uracil used in hplc calibration?
higly unretainable and has high absorption at 260 nm
Why uracil is used in hplc calibration?
higly unretainable and has high absorption at 260 nm
What are the solvents used in gas chromatography calibration?
Tetrahydrofuran, Acetone and dimethylsulfoxide
What are the HPLC Calibration parameters and elaborate it?
1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Why internal standard you used in hplc method?
Internal standard can be used for calibration by plotting the ratio of the analyte signal to the internal standard signal as a function of the analyte concentration of the standards. This is done to correct for the loss of analyte during sample preparation or sample inlet.
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
When calibration required?
Calibration is required during installation of new instrument to check whether the instrument works as per specifications or not. Calibration is required when instrument is shifted from one location to another. Calibration of instrument is done when instrument is used again after maintenance. Some instruments such as weighing balance are calibrated daily while some instruments or equipments such as HPLC, GC, etc., are calibrated or validated at specified interval as per standard operating procedure (SOP).
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
How does one apply the HPLC princple?
The HPLC principle is used to separate liquid mixtures into individual parts. It's frequently used in several field such as medicine and law. HPLC is specifically used in sports to test for performance enhancing drugs in urine.
