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DNA is negatively charged and a current is running through the gel with the positive pole and the foot of the gel run, so the DNA migrates from the head of the run towards its oppositely charged pole.

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14y ago
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12y ago

DNA molecules are negatively charged. Therefore when they are run using gel electrophoresis they move towards the positive electrode. (This is because positive and negative attract each other).

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11y ago

DNA is a negatively charged molecule overall. When DNA fragments are placed in an acrylimide gel, an electric field is applied to the gel. The DNA fragments are "pulled" by the electrostatic attraction between the negative charges on the fragments and the positive charge at the far end of the plate. Based upon the size of the holes in the gel, different length fragments move a different distance, resulting in the characteristic banding pattern associated with a DNA fingerprint.

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12y ago

DNA fragment move through the gel to get toward the positive poles this is called Buffer solution

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14y ago

DNA is negatively charged. When loaded on to the gel, the DNA moves toward the positive electrode.

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12y ago

Toward the positive electrode

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Q: What cause the DNA fragments to move through the gel?
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The rate at which large DNA fragments move through the electrophoretic gel is?

the answer to this question is that, its greater than the rate at which small DNA fragments move through the same apparatus


What is used to separate DNA fragments by size?

Electrophoresis. Restriction enzymes are used to cut DNA into fragments. Solutions containing these fragments are placed on the surface of a gel to which an electric current is applied. The electric current causes the DNA fragments to move through the gel. Because smaller fragments move more quickly than larger ones, this process separates the fragments according to size.


Does gel electrophoresis seperate dna fragments?

dna fragments are negatively charged is the answer for apex.


Explain how an agarose gel can separate DNA fragments of different lengths.?

The separation of DNA fragments is based on size. When a DNA sample is run in a gel (electrophoresis), the lighter fragments migrate faster than the heavier (longer) fragments under the influence of an electric current. At the and of the process, the shorter fragments are found at the terminal end of the gel and the longer fragments closer to the origin


Why are D1S80 fragments separated by gel electophoresis?

Fragments are separated by gel electrophoresis because of their differing sizes. DNA is negatively charged, so will migrate through the gel towards the positive electrode. The smaller fragments are able to move through the gel more quickly than the larger fragments - which means they separate based on their size.


Gel electrophoresis separates DNA fragments on the basis of differences in their?

Length. DNA has a natural negative charge - and so will move towards the positive electrode. Larger fragments move more slowly than shorter ones - so the sizes of fragments can be determined.


Why is the largest DNA fragment band found closest to the well in with it was placed?

Because of the molecular weight of DNA, the larger fragment DNA is heavier and cannot move fast through the gel and found near to well whereas the small fragments with small molecule weight can move fast through the gel pores


How does electrophoresis work?

Electrophoresis for nucleic acids (RNA and DNA) works by separating segments by their size. This is possible because RNA and DNA are negatively charged, so will move towards the positive charge applied to one end of the gel. The different segments separate because small fragments of RNA or DNA are able to move more quickly through the gel than larger fragments.


What determines the distance of the DNA fragments?

By the size of the DNA molecule. longer DNA molecules move slower, and shorter DNA molecules move faster!


After DNA is cut with a restriction enzymes how is the mixture of DNA fragments sorted?

Through the process of gel electrophoresis.


Where do you get the DNA fragments for Gel electrophoresis?

The DNA fragments comes from the method of DNA isolation.


What do DNA bands represent in the agarose gel electrophoresis?

Each band represents a piece of DNA. The extent to which they move through the gel has to do with the fragment's electrophoretic mobility. The lighter the molecule in general the faster it can move through the gel. Usually when performing a gel electrophoresis one would use markers. These markers would be of known molecular weight and would allow you to compare your DNA fragments and find approximate molecular weights.