PBS is used as a cell culture media because every live-system nedds a ionic concentration in your environment. PBS contains phosphate and NaCl, and its a "basic" media culture, and you can add another components, like antibiotics, hormones or enzymes, for example. Remember that cells haves a membrane that exchanges substances with the environment all the time. Therefore, it needs an adjusted environment. Water can´t offer this environment to the cell growth, because you only can use distilled water, to prevent contaminations. Like this way, however, you "lost" every ion, and so the cell don´t have the "perfect" media to growth. Cell culture medias, like PBS and many others (IMDM, for example), is nothing more than distilled water added by ideal ions to the cell life. Each type of cell "prefers" a cell culture media to growth.
Phosphate-buffered saline provides exactly what it's name implies: buffer (so that the pH stays approximately constant) and just as many ions per unit volume as the inside of a cell (so that the cells don't swell or shrink).
Cell culture media has trypsin neutralizers, so when you wash with PBS beforehand you are washing away these factors, making trypsin active.
A cell culture is where cells are grown under controlled conditions.
Cell culture is a nearly universally used technique to grow material for biological testing. In cell culture, an original population of seed cells divide under tightly controlled conditions to propagate the culture. All of the daughter cells from the original population are clones.
to culture any organ , tissue or cell from single cell called cell culture. to grow any tissue or organ from a tissue called tissue cultured. and formation of any organ from source organ is called organ culture. in short according to the source of culture any cultured are named.
=) hi
Cell culture media has trypsin neutralizers, so when you wash with PBS beforehand you are washing away these factors, making trypsin active.
What cell walls are you using? In mammalian cell culture the reason you would use Ca/Mg free PBS is to help remove them from the surface they are attached to. The adherens proteins that adherent cells use to stick to whatever they are stuck to requires Ca2+ and Mg2+. Therefore if you simply want to wash the cells you have to use a buffer WITH Ca2+ and Mg2+.
A cell culture is where cells are grown under controlled conditions.
A cell culture is where cells are grown under controlled conditions.
A cell culture is where cells are grown under controlled conditions.
Cell culture is a nearly universally used technique to grow material for biological testing. In cell culture, an original population of seed cells divide under tightly controlled conditions to propagate the culture. All of the daughter cells from the original population are clones.
The stem cell "culture" is the medium in which the stem cell is able to divide and grow.
Martha on PBS Kids is Martha on PBS Kids.
to culture any organ , tissue or cell from single cell called cell culture. to grow any tissue or organ from a tissue called tissue cultured. and formation of any organ from source organ is called organ culture. in short according to the source of culture any cultured are named.
treated means, the cell culture is treated with some agents/compounds/drugs/even antibiotic etc.
=) hi
PBS Kids 900:000 AM PBS Kids Sprout (satallite and Verizon) 789:087 AM PBS 200:000 AM