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What is PBS in cell culture?

Updated: 8/11/2023
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Ilikeu2

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16y ago

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PBS is used as a cell culture media because every live-system nedds a ionic concentration in your environment. PBS contains phosphate and NaCl, and its a "basic" media culture, and you can add another components, like antibiotics, hormones or enzymes, for example. Remember that cells haves a membrane that exchanges substances with the environment all the time. Therefore, it needs an adjusted environment. Water can´t offer this environment to the cell growth, because you only can use distilled water, to prevent contaminations. Like this way, however, you "lost" every ion, and so the cell don´t have the "perfect" media to growth. Cell culture medias, like PBS and many others (IMDM, for example), is nothing more than distilled water added by ideal ions to the cell life. Each type of cell "prefers" a cell culture media to growth.

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16y ago

Phosphate-buffered saline provides exactly what it's name implies: buffer (so that the pH stays approximately constant) and just as many ions per unit volume as the inside of a cell (so that the cells don't swell or shrink).

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