Why isn't the oil immersion lens used in the hanging-drop procedure?

Answer 1

The purpose to use oil is to make it clear in the microscope.

Oil is the quintessential "tool" to make oil immersion magnification possible. Oil has the same refractive index as glass, therefore the light is not bent by the air that would otherwise be in the oil's place.

Answer 2

the hanging drop procedure uses a thicker slide, which could crack if the oil immersion lens is used. My bio professor worte: hanging drop slides asre too thick to use under oil.

Answer 3

In a compound microscope the lens appropriate for the size of the specimen must be used. As you move from lens to lens 4x, 10x, 40x, 100x, the object is magnified larger and larger and the field of view of the object gets smaller and smaller. Oil is only used in cases where very fine detail of microscopic objects is necessary such as viewing bacteria or zooming in on what is inside an individual animal or plant cell. Oil is only used on the oil immersion lens (100x) due to the fact that it has the smallest opening for light and placing oil on the specimen helps bend more light rays up into the lens to obtain a clearer image.

Answer 4

The purpose to use oil is to make it clear in the microscope.

Oil is the quintessential "tool" to make oil immersion magnification possible. Oil has the same refractive index as glass, therefore the light is not bent by the air that would otherwise be in the oil's place.

Answer 5

because both the wet mount and the immersion oil have almost the same refractive index, so we dont need extra refraction