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Why liquid paraffin is prefer while using oil immersion objective?
Wiki User
∙ 10y ago
Liquid paraffin, also known as mineral oil, is commonly used with oil immersion objectives in microscopy because it has a refractive index similar to that of glass and the immersion medium used in the objective. This reduces the loss of light due to refraction and increases the numerical aperture, allowing for better resolution and increased imaging quality. Additionally, liquid paraffin has a high viscosity which helps to prevent the formation of air bubbles and ensures a stable imaging medium.
Wiki User
∙ 10y agoLiquid paraffin is a preffered immersion oil because althogh having a lower RI than cedar oil it is less likely to result in damage to the objective lens. Synthetic oils have better characteristics but are expensive.
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