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Why the cell do not contain plasmid if they not grow in antibiotics?
Wiki User
∙ 9y ago
If you do not give antibiotics, the plasmid may lost after a few generation. it is because bacteria thinks that the extra DNA (plasmid) is not needed as the antibiotic is not present. That is the reason for keeping the culture always in antibiotic selection pressure.
Wiki User
∙ 9y ago
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Why do the cells transformed with pUC18 and plasmid lux grow in the presence of ampicillin?
they both contain an ampicillin resistent gene
How will you identify cells carrying the recombinant plasmid?
The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
What is one way to determine whether a bacterial culture has received a recombinant plasmid?
The plasmid have a "reporter gene" inside it, generally resistance to specific antibiotic. the plasmid is transformed into bacteria that don't have resistance to that specific antibiotic drug, and than the cultured on a petri-dish that contain the antibiotic drug. Only bacteria that had receive the plasmid will have resistance and grow, all the other will die.
What important molecules does the nucleas contain?
The nucleus contains the DNA for that cell. Without the DNA, the cell cannot grow and develop fully.
What problems occur as cell grow?
it may not be able to contain itself if it gets too large
Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow where?
the ampicillin broth and the nutrient broth
What is cryptic contamination?
A cryptic contamination is a possible result of using antibiotics in a cell culture. Some microorganisms like mycoplasma are resistant to antibiotics and will still grow slowly in the presence of the antibiotic. This means that a cell culture can be highly contaminated but does not show any visible effect except false results when the experiment is evaluated
You have propagated a vector in a non expression host and isolated the plasmid How can you confirm whether the plasmid that you got is exactly the vector which you propagated?
Check the size in agarose gel, extract it from the gel then purify it and grow it on selective plate.
Bacterial cells which have been transformed with a plasmid are allowed to grow so that they the plasmids everytime they divide?
for plate answer is D
Why should you take your antibiotics?
If you dont take all of your antibiotics the bacteria will continue to grow and its DNA will change so it will become resistent to the antibiotics
The takes up the plasmid. It now contains the human gene?
I think I know the answer... it's 5
What is the significance of E. coli DH5 alpha?
E. Coli DH5 alpha cells are mainly of value to scientific research. They are frequently used for transformation of plasmid DNA, which is a research tool for amplifying the amount of plasmids. One can insert a gene of interest into a plasmid but the yield of this process is limited. If you were then to insert this plasmid into a living cell, your plasmid would be copied with every division of that cell. Bacteria normally host plasmids and are not too picky about it so one more is usually not a problem. Since bacteria like E. coli are really easy to grow, maintain and store, the DH5 alpha strain is perfect. As far as technical details about the strain go: DH5 alpha have been made deficient in some genes, which protects foreign DNA for instance. endA1 gene in DH5 alfa is modified (mutated) so that the intracellular endonuclease it code is inactive that degrades plasmid DNA in many preparations
