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Why the first restriction endonuclease is known as Hind2 and not Hind1?
Restriction enzymes are named based on the organism in which they were discovered. For example, the enzyme Hind III was isolated from Haemophilus influenzae, strain Rd. The first three letters of the name are italicized because they abbreviate the genus and species names of the organism. The fourth letter typically comes from the bacterial strain designation. The Roman numerals are used to identify specific enzymes from bacteria that contain multiple restriction enzymes. Typically, the Roman numeral indicates the order in which restriction enzymes were discovered in a particular strain.There are three classes of restriction enzymes, labeled types I, II, and III. Type I restriction systems consist of a single enzyme that performs both modification (methylation) and restriction activities. These enzymes recognize specific DNA sequences, but cleave the DNA strand randomly, at least 1,000 base pairs(bp) away from the recognition site. Type III restriction systems have separateenzymes for restriction and methylation, but these enzymes share a common subunit. These enzymes recognize specific DNA sequences, but cleave DNA at random sequences approximately twenty-five bp from the recognition sequence. Neither type I nor type III restriction systems have found much application in recombinant DNA techniques.Type II restriction enzymes, in contrast, are heavily used in recombinant DNA techniques. Type II enzymes consist of single, separate proteins for restriction and modification. One enzyme recognizes and cuts DNA, the other enzyme recognizes and methylates the DNA. Type II restriction enzymes cleave the DNA sequence at the same site at which they recognize it. The only exception are type IIs (shifted) restriction enzymes, which cleaveDNA on one side of the recognition sequence, within twenty nucleotides of the recognition site. Type II restriction enzymesdiscovered to date collectively recognize over 200 different DNA sequences.
What type of science is cloning?
= Genetics =
Substance that enzyme act upon?
They are known as reactants or substrates.
What is substrate concentration?
it is a type of enzyme.
What does a repressor do in the enzymes active site?
Repressors bind to the silencers in the DNA to block the RNA polymerase from binding to the promoter of the gene to reduce gene expression, not really binding to enzymes active sites I think what you meant was "what does an inhibitor do to the enzymes active site"? In which case, it depends on the type of inhibitor. A competitive inhibitor has a structure similar to the substrate, hence would bind to the active site as well, competing with the substrate for the enzyme active sites, decreasing enzymatic activity. A non-competitive inhibitor binds to the allosteric site of the enzyme, causing a structural change in the enzyme active site shape. Hence the enzyme would not be able to bind to the original substrate, so enzymatic activity comes to a halt for the enzymes that are bound by the non-competitive inhibitors
What type of the enzyme that cuts the bonds of DNA molecule at the origin of replication?
restriction endonucleases
What type of enzyme is used to fragment dna?
restriction enzymes are used to cut DNA.
What is orchid cloning?
Cloning is a type of asexual reproduction.
Function of restriction enzymes?
They cut strands of DNA at specific sites.
Why the first restriction endonuclease is known as Hind2 and not Hind1?
Restriction enzymes are named based on the organism in which they were discovered. For example, the enzyme Hind III was isolated from Haemophilus influenzae, strain Rd. The first three letters of the name are italicized because they abbreviate the genus and species names of the organism. The fourth letter typically comes from the bacterial strain designation. The Roman numerals are used to identify specific enzymes from bacteria that contain multiple restriction enzymes. Typically, the Roman numeral indicates the order in which restriction enzymes were discovered in a particular strain.There are three classes of restriction enzymes, labeled types I, II, and III. Type I restriction systems consist of a single enzyme that performs both modification (methylation) and restriction activities. These enzymes recognize specific DNA sequences, but cleave the DNA strand randomly, at least 1,000 base pairs(bp) away from the recognition site. Type III restriction systems have separateenzymes for restriction and methylation, but these enzymes share a common subunit. These enzymes recognize specific DNA sequences, but cleave DNA at random sequences approximately twenty-five bp from the recognition sequence. Neither type I nor type III restriction systems have found much application in recombinant DNA techniques.Type II restriction enzymes, in contrast, are heavily used in recombinant DNA techniques. Type II enzymes consist of single, separate proteins for restriction and modification. One enzyme recognizes and cuts DNA, the other enzyme recognizes and methylates the DNA. Type II restriction enzymes cleave the DNA sequence at the same site at which they recognize it. The only exception are type IIs (shifted) restriction enzymes, which cleaveDNA on one side of the recognition sequence, within twenty nucleotides of the recognition site. Type II restriction enzymesdiscovered to date collectively recognize over 200 different DNA sequences.
The gene for ABO blood type encodes an enzyme that adds sugar molecules to lipids on the surface of red blood cells?
It's true.
How bacteria can protect themselves from attack by virus?
It produce some type of anzyme callet restriction enzyme that cut the foreing DNA of the virus that have been previoslly integrated into the host genome.
What gene causes albinism?
Ocular albinism (OA1) is caused by a genetic defect on chromosome 11 of the GPR143 gene. Oculocutaneous albinism type 2 (OCA2 or P gene albinism) results from a genetic defect on chromosome 15 in the P protein that helps the tyrosinase enzyme to function. Oculocutaneous albinism type 3 (OCA3) is rarely described and results from a genetic defect on chromosome 9 in TYRP1, a protein related to tyrosinase. Oculocutaneous albinism type 4 (OCA4) results from a genetic defect in chromosome 5 at position 13.3, in the SLC45A2 protein that helps the tyrosinase enzyme to function.
What type of science is cloning?
= Genetics =
Which type of enzyme removes hair from skin?
The Enzyme Skin Peel is the type of enzyme that removes hair from the skin.
What type of enzyme is plasmin?
Blood enzyme
A plasmid a foreign gene and recombinant DNA would be associated with what type of organism?
A plasmid is a double stranded cirucular DNA, used as a vector in cloning. A gene of interest can be ligated into the this to form a chimeric DNA or rDNA. This can be transformed to a bacteria for propagation of the clones (you can amplify them by these transformed bacteria).
