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No, of course it is possible that one could start out with an already diluted sample in the first step. So then perhaps in the third sector some isolated colonies may form, because the amount of bacteria have been diluted enough.
No. It depends on the number of bacteria present in the initial sample. If the number of bacteria in the initial sample are limited, you may get isolated colonies in the first streak. If the number of bacteria in the sample are high, it may take several streaks before the sample is diluted to the point where isolated colonies are evident.
Quintant.
FMCG
A quadrant is a sector that equals 1/4 of a circle.
A sector has a fixed size; it will always be 512 bytes. A cluster can be as small as one sector, and can be as big as it needs to be.
Motexx (located in the Motexx system within the Varvenna sector in the Colonies).
Neuvia (located in the Neuvia system within the Belnar sector in the Colonies).
Norclune (located in the Norclune system within the Wyloff sector in the Colonies).
Razia (located in the Razia system within the Varvenna sector in the Colonies).
Zhann (located in the Zhann system within the Duluur sector in the Colonies).
Perthrillia (located in the Perthrillia system within the Phu sector in the Colonies).